1. ‣ASTM D5511-11
   

1. ‣Standard Test Method for Determining Anaerobic Biodegradation of Plastic Materials Under High-Solids Anaerobic-Digestion Conditions Considering Gas Evolution
   

2. 
   

1. ‣Monitors the level of microbial activity of anaerobic organisms (inoculum) as they are exposed to test material.
   

2. ‣If the inoculum recognizes the test material as a nutrient source, then an increase in activity is recorded in the form of gas evolution.
   

3. ‣The evolved gas is monitored for volume and composition.
   

4. •    Volume by water displacement
   

5. •Composition by Gas Chromatograph (CO2 & CH4)
   

6. ‣Samples are setup in triplicate and and run against three or four triplicate controls.
   

7. •    Positive Control - Cellulose
   

8. •    Negative Control - Polyethylene
   

9. •    Inoculum Control - Background
   

10. •    Negative Untreated Control - Optional
    

11. ‣Results are reported in percent biodegraded per time tested.
    

3. 
   

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